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. 2012 May 3;8(5):e1002669. doi: 10.1371/journal.pgen.1002669

Figure 4. Overexpression of HsfA2 complements the heat-sensitive phenotype of rps1 mutant plants.

Figure 4

(A) Phenotypes of WT, rps1 and rps1 with a 35S:HsfA2 cDNA transgene as 21-d-old plants. (B) to (C) HsfA2 mRNA levels in leaves of the plants described in (A) were analyzed by RT-PCR (B) and qRT-PCR (C). Actin2 was used as the internal standard. Error bars indicate standard deviations of three technical replicates, and the results were consistent in three biological replicates. (D) to (F) Heat-challenged phenotypes of different genotypes as described in (A) as examined with young seedling (D), detached leaf (E) and whole plant (F) assays performed as described in Materials and Methods.