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. 2012 May 3;8(5):e1002669. doi: 10.1371/journal.pgen.1002669

Figure 7. Disturbance of RPS1 expression destabilizes thylakoid membranes.

Figure 7

(A) Estrogen-induced variegated phenotypes of transgenic seedlings harboring estrogen-inducible RPS1-RNAi constructs. (B) RPS1 mRNA levels in leaves of the transgenic plants as described in (C) were examined by RT-PCR (left) and qRT-PCR (right). For qRT-PCR analysis, Actin2 was used as the internal standard. Error bars indicate standard deviations of three technical replicates, and the results were consistent in three biological replicates. (C) 35S:RPS1 cosuppressed lines showing a variegated phenotype. (D) RPS1 protein levels in green and white sectors excised from leaves of cosuppressed lines were examined by western blot analysis with an RPS1 polyclonal antibody. Equal protein loading was confirmed with antiserum against α-Tublin. (E) Cross-sectional analysis of thylakoid membranes in chloroplasts from the green sector, transition section, and white sector of a leaf excised from 35S:RPS1 cosuppressed line CS1 by transmission electron microscopy (TEM). Bars = 1 µm.