Figure 4. Diet-induced shift in energy storage is not sufficient to induce sleep partitioning.

(A,B) A metabolite panel was tested in control (yw) flies that had previously been fed 2.5% (LS) or 30% (HS) sucrose on a 2.5% yeast base food for 6 days during activity monitoring. There was a significant change in triglyceride (TAG) and a smaller change in protein levels with no significant effect on glycogen (Gly), glucose (Glu), or trehalose. (C) Flies carrying the imprecise P-element excision deletion Lsd-2⁵¹ had reduced triglyceride∶protein levels relative to precise excision Lsd-2^rev controls (inset) but retained a significant sleep response to diet with no diet∶genotype interaction (two-way ANOVA). Error bars represent mean +/− SEM for each group. *** = p<0.001, ** = p<0.01,* = p<0.05 for all statistical calculations. Error bars represent mean +/− SEM for each group. *** p<0.001, ** p<0.01,* p<0.05 for all statistical calculations. Significance values for t-tests between dietary conditions are shown above each set of bars and significance values for two-way ANOVA are shown above the graph, where applicable.