Skip to main content
. 2012 May 3;8(5):e1002667. doi: 10.1371/journal.ppat.1002667

Figure 4. Endotoxic activity of C. canimorsus (Cc) LPS, lipid A (LA) or lipid A-core (LA-core) and contribution of the LPS core to endotoxicity.

Figure 4

(A–B) Dose-response curve of purified lipid A, LA-core or LPS. Samples were assayed for TLR4 dependent NFκB activation with HEKBlue human TLR4 cells. (C–D) Purified lipid A, LA-core or LPS samples were assayed for induction of TNFα release by human THP-1 macrophages. (E) Purified LPS samples or lipid IVA were assayed for induction of IL-6 release by canine DH82 macrophages. (F) Dose-response curve of NFκB activation by lipid A or LPS. Purified lipid A or LPS samples were assayed for TLR4-dependent NFκB activation with HEKBlue human TLR4 cells. The C. canimorsus lipid A stock solution was pretreated with either 0.1% TEN or 50% DMSO and sonication to increase its solubility in water/buffer. Identical concentrations of DMSO or TEN were added to E. coli O111 LPS as a control. Data were combined from n = 3 independent experiments, error bars indicated are standard error of the mean.