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. 2012 May 3;8(5):e1002693. doi: 10.1371/journal.pgen.1002693

Table 1. Mass-spectrometric analysis of IDN2-6xMyc affinity purification.

Accession number Protein Mascot score MW (Da) 1Matched queries 2Matched peptides 3Unique matches
Total IDN2-Myc purified proteins in WT
IPI00524938 AT3G48670 IDN2 2341 74778 118 45 118
IPI00523067 AT1G15910 IDP1 1480 72590 68 37 53
IPI00542085 AT4G00380 IDP2 444 73119 34 24 19
The ∼80 KDa band from IDN2-Myc purified proteins in WT
IPI00523067 AT1G15910 IDP1 2092 72590 135 54 88
IPI00542085 AT4G00380 IDP2 612 73119 59 30 12
IPI00524938 AT3G48670 IDN2 528 74778 33 26 33
The ∼80 KDa band from IDN2-Myc purified proteins in ros1idn2
IPI00523067 AT1G15910 IDP1 1891 72590 76 35 46
IPI00542085 AT4G00380 IDP2 606 73119 37 20 7
The ∼80 KDa band from IDN2E600Q-Myc purified proteins in ros1idn2
IPI00523067 AT1G15910 IDP1 829 72590 26 21 20
IPI00542085 AT4G00380 IDP2 208 73119 10 10 4
The ∼80 KDa band from IDN2W616A-Myc purified proteins in ros1idn2
IPI00523067 AT1G15910 IDP1 458 72590 25 15 19
IPI00542085 AT4G00380 IDP2 143 73119 10 8 4

The protein extracts were prepared from the IDN2-6xMyc transgenic plants in the wild-type and ros1idn2-4 mutant backgrounds and used for affinity purification of the IDN2-6xMyc-containing complex. Either total purified proteins or the ∼80-KDa band were subjected to mass-spectrometric analysis. Mascot score of each protein is shown. 1) The number is the total number of mass spectra matched to the corresponding protein, including the redundant ones that match to the same peptides. 2) The number is deduced from the “Matched queries” by removing the redundant peptides. 3) The number is calculated from “Matched queries” by removing the overlapped peptide sequences among its homologous protein family.