Figure 2.

Bryostatin 1 induced upregulation of CD22 depends on PKC-β activity. (A) CD22 upregulation by bryostatin 1 is blocked by the PKC inhibitor enzastaurin (10 μM) but not by the ERK inhibitor PD98059 (50 μM). Cells were pre-treated with inhibitors for 120 min and then cultured with bryostatin 1 (1 ng/mL) for 24 h. The same results were obtained in 3 out of 4 individual experiments. (B) Primary CLL cells were exposed to either 1 ng/mL or 50 ng/mL bryostatin 1 for 24 h. Cells were then harvested and analyzed for the expression of PKC-βII or PKC-ɛ. To indicates cell lysates from CLL cells freshly isolated from peripheral blood. Results from 3 experiments revealed the same results.