Figure 7. A reduction in COX-2 level upon treatment with N-acetyl cysteine.

We exposed parental SUM149-Luc and Gln-ind cell lines, both growing in a medium with glutamine, to N-acetyl cysteine for 24 hours before subjecting them to western blotting using equal volumes of cell lysates. Since β-actin was not a reliable indicator of protein loading, we ran a gel in parallel and stained it with Coomassie blue. Lane numbers 1–6 at the bottom in left panel correspond to lane numbers 1–6 in the right panel.