Figure 10.

(A) Higher adaptability/survival of Gln-ind versus parental cells in the absence of serum. Cell cultures were deprived of serum for 25 days, and resulting colonies were stained with crystal violet. (B) Higher anchorage-independence of Gln-ind versus parental cells. Twelve thousand cells were cultured in Gln-containing medium with 0.35% low-melt agarose for 23 days and the resulting colonies were stained with crystal violet.