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. 2012 May 3;7(5):e36683. doi: 10.1371/journal.pone.0036683

Figure 2. Secreted and cell-associated AChE activity in control and transfected cells.

Figure 2

Increase of AChE activities following AChE-transfection is diminished by cultivation on laminin-1, in particular so if the PRiMA anchor is co-transfected. R28 cells were transfected with E6-AChE, R395C AChE, PRiMA and GFP, and AChE activity in cell lysates (A) and medium (B) was determined. Control clones produced by transfecting with empty vector or GFP showed activity levels similar to those of untransfected cells. Therefore, the GFP expressing cell line was used as control for further experiments. Results are given as means ± standard deviation for at least five separate experiments. * p<0.05; *** p<0.001. All activities were significant increased when compared with control cells.