Figure 4. Protection induced by immunization with recombinant R4-GST or by the whole recombinant Spr1875 protein fused to a biotinylated tag (Spr1875-BIO).
(A) Groups of CD1 mice were immunized with R4-GST or with GST, used as a negative control, and challenged with the D39 strain (1×105 CFU). Results represent cumulative data from 3 experiments; n = 28, total number of animal per each group. (B) Groups of CD1 mice were immunized with Spr1875-BIO or with CAT-BIO, used as a negative control, and challenged with the D39 strain (1×105 CFU). Results represent data from one experiment involving 14 animals per group. *, statistically different (p<0.05) from mice challenged with D39 strain, as assessed by Kaplan-Meier estimator of survival.