Figure 2. ITC duplex assay equivalence testing.

All three ITC duplex assays were tested in the presence of same amount of target and ITC template and 500 ng naïve gDNA. Five copies of pSSV9-MD2-cmEPO plasmid and ITC^cmEPO template were amplified using the ITC^cmEPO duplex assay. 10 copies of pShuttle-CAG-hEPO-pA plasmid and ITC^hEPO template were amplified using the ITC^hEPO duplex assay. 10 copies of pSSV9-MD2-cmEPO plasmid and ITC^CMV template were amplified using the ITC^CMV duplex assay. Each reaction was repeated 15 times. Similarities in mean Ct values were analyzed statistically as described in Materials and Methods.