Table 1. Individual assay linearity and efficiency.
Assay | Linearity (R2) | Linearity (R2) | Efficiency (%) | Efficiency (%) |
in the absence of 500 ng gDNA | in the presence of 500 ng gDNA* | in the absence of 500 ng gDNA | in the presence of 500 ng gDNA | |
cmEPO | 0.992 | 0.989 | 95 | 82 |
ITCcmEPO | 0.989 | 0.998 | 91 | 86 |
hEPO | 0.999 | 0.998 | 92 | 88 |
ITChEPO | 0.996 | 0.993 | 89 | 86 |
CMV | 0.996 | 1.000 | 99 | 97 |
ITCCMV | 0.997 | 0.989 | 98 | 94 |
macaque gDNA with cmEPO and ITCcmEPO assays, and human gDNA with hEPO, ITChEPO, CMV and ITCCMV assays.