Table 2. Individual assay sensitivity.
| Assay | Positive Control | Mean* Copy Number (s.e.) | Mean* Ct (s.e.) | CV (%) of Ct |
| cmEPO | 5 copies pDNA with 500 ng gDNA (macaque) | 6.7 (2.5) | 37.24 (0.22) | 2.2 |
| 5 copies pDNA with TE | 5.9 (3.4) | 37.47 (0.28) | 2.9 | |
| ITC cmEPO | 5 copies ITC template with 500 ng gDNA (macaque) | 6.5 (2.7) | 37.22 (0.24) | 1.5 |
| 5 copies ITC template with TE | 5.5 (3.8) | 37.14 (0.31) | 3.2 | |
| hEPO | 10 copies pDNA with 500 ng gDNA (human) | 9.6 (3.3) | 36.74 (0.14) | 1.5 |
| 10 copies pDNA with TE | 10.8 (2.8) | 36.45 (0.10) | 1.1 | |
| ITChEPO | 10 copies ITC template with 500 ng gDNA (human) | 11.8 (3.9) | 36.55 (0.14) | 1.6 |
| 10 copies ITC template with TE | 11.1 (2.4) | 36.73 (0.10) | 0.9 | |
| CMV | 10 copies pDNA with 500 ng gDNA (human) | 11.9 (5.3) | 36.29 (0.18) | 2.0 |
| 10 copies pDNA with TE | 11.1 (4.1) | 36.35 (0.14) | 1.5 | |
| ITCCMV | 10 copies ITC template with 500 ng gDNA (human) | 10.7 (4.3) | 36.64 (0.16) | 1.7 |
| 10 copies ITC template with TE | 11.8 (4.0) | 36.55 (0.13) | 1.4 |
*
From fifteen replicates of plasmid (pDNA) or ITC template. Standard error, s.e., (in parenthesis).