Table 6. Human EPO cDNA detection in the presence of different naïve human gDNA samples.

Human gDNA	5∶10*			10∶10**			20∶10***
	Mean§ hEPO Ct	Mean ITChEPO Ct	P-Value	Mean§ hEPO Ct	Mean ITChEPO Ct	P-Value	Mean§ hEPO Ct	Mean ITChEPO Ct	P-Value
1	37.36 (0.84)	36.25 (0.53)	0.0368	36.80 (0.41)	36.70 (0.83)	0.8853	35.36 (0.46)	36.95 (0.50)	0.0482
2	38.03 (0.40)	36.25 (0.24)	0.0052	36.88 (0.32)	36.99 (0.58)	0.8663	35.17 (0.39)	36.41 (0.14)	0.0168
3	37.45 (0.85)	36.39 (0.38)	0.0253	36.94 (0.44)	37.14 (0.83)	0.7840	35.18 (0.44)	37.46 (0.59)	0.0143
4	37.62 (0.33)	36.24 (0.10)	0.0040	36.91 (0.35)	37.00 (0.79)	0.8924	35.34(0.45)	36.97 (0.50)	0.0425

^§From five replicates of pShuttle-CAG-hEPO-pA plasmid DNA spiked into 500 ng naïve human gDNA. Standard error (in parenthesis).

^*5 copies of plasmid and 10 copies of ITC template.

^**10 copies of plasmid and 10 copies of ITC template.

^***20 copies of plasmid and 10 copies of ITC template.

The number of cycles of the real-time PCR program was set to 40.