Table 7. CMV promoter detection in the presence of different naïve human gDNA samples.
| Human gDNA | 5∶10* | 10∶10** | 20∶10*** | ||||||
| Mean† CMV Ct | Mean ITCCMV Ct | P-Value | Mean† CMV Ct | Mean ITCCMV Ct | P-Value | Mean† CMV Ct | Mean ITCCMV Ct | P-Value | |
| 1 | 38.88 (0.35) | 36.27 (0.08) | <0.0001 | 36.77 (0.08) | 36.62 (0.12) | 0.3259 | 36.21 (0.09) | 36.65 (0.11) | 0.0172 |
| 2 | 38.37 (0.32) | 36.70 (0.12) | 0.0011 | 36.56 (0.14) | 36.97 (0.15) | 0.3333 | 36.11 (0.11) | 36.69 (0.08) | 0.0025 |
| 3 | 38.55 (0.42) | 36.82 (0.13) | 0.0045 | 36.78 (0.07) | 36.74 (0.13) | 0.7807 | 36.23 (0.14) | 36.91 (0.13) | 0.0081 |
| 4 | 38.83 (0.32) | 36.73 (0.18) | 0.0005 | 36.67 (0.12) | 36.67 (0.08) | 0.9355 | 36.06 (0.23) | 36.71 (0.04) | 0.0183 |
†
From five replicates of pSSV9-MD2-cmEPO plasmid DNA spiked into 500 ng naïve human gDNA. Standard error (in parenthesis).
*
5 copies of plasmid and 10 copies of ITC template.
**
10 copies of plasmid and 10 copies of ITC template.
***
20 copies of plasmid and 10 copies of ITC template.
The number of cycles of the real-time PCR program was set to 40.