Table 2.

Immunohistochemistry techniques

Antibody	Company	Clone	Dilution	Antigen retrieval	Incubation
NASP	UNC School of Medicine	R8547(6)	1:100	EDTA steam	2 h
RCAS1	Medical & Biological Laboratories, Woburn, MA	22-1-1	1:500	Citrate steam	2 h
eIF5A	Abcam, Cambridge, MA	LF5A2	1:2000	CC1 automated	32 min
NBS1	Cell Signaling, Beverly, MA	p95/NBS1	1:50	Citrate steam	1 h
MRE11	AbCam, Cambridge, MA	ab214	1:4000	CC1 automated	32 min
RAD50	AbCam, Cambridge, MA	ab89	1:2000	CC1 automated	20 min
Her2	Dako, Carpenteria, CA	c-erbB2	1:150	Citrate steam	2 h
p53	Ventana, Tucson, AZ	DO7	prediluted	CC1 automated	32 min