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. 2012 May 3;7(5):e36632. doi: 10.1371/journal.pone.0036632

Figure 6. CD36 and PAFR cooperatively mediate FITC-oxLDL uptake and IL-8 production.

Figure 6

HEK 293t cells were transiently transfected with hCD36 and/or hPAFR, as described in methods (A–B). Transfected cells were incubated with FITC-oxLDL (30 µg/mL) for 1 h at 37°C. Cells were washed and fixed with paraformaldehyde 4%. Fluorescence was visualized by microscopy (C). Transfected cells were stimulated with oxLDL (30 µg/mL) and the IL-8 concentration in the supernatant was measured after 24 h (D). * p<0.05 comparing with cDNA3 plasmid control transfected cells.