Figure 4. Adoptively transferred OT-I CD8⁺ T cells are deleted in SM-Ova mice.

(A) 5×10⁶ purified CD8⁺CD45.1⁺ OT-I cells were adoptively transferred into SM-Ova or control B6 mice. Recipients were euthanized 2 days or 11 weeks after to determine the percentages (indicated in the representative flow cytometry panels on top) and total numbers (represented below in the bar graphs) of CD8⁺CD45.1⁺ OT-I cells recovered from the LNs and spleen of recipient mice. (B) 5×10⁶ CFSE-labeled CD8⁺CD45.1⁺ OT-I cells were injected i.v. into SM-Ova or B6 control mice. Proliferation of gated CD8⁺CD45.1⁺ cells was evaluated in the LN and spleen of recipient mice by flow cytometry 7 or 15 days after their adoptive transfer. Representative profiles are shown and the indicated numbers correspond to the percentage of gated cells that have diluted CFSE upon cell division. Data are representative of 2 independent experiments performed with 10 mice per group.