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. 2012 May 3;7(5):e36291. doi: 10.1371/journal.pone.0036291

Table 1. EQA results of the 28 participant laboratories in the molecular diagnosis panel.

Sample ID #2 #9 #12 #4 #14 #10 #5 #13 #1 #6 #11 #3 #8 #7
Viral load in sample 3×106 3×105 3×104 3×103 3×102 104 103 2×104 2×103 69 NEG NEG NEG NEG
Lab. no. RT-PCR technique 17D 17D 17D 17D 17D Brazil Brazil Ivory Coast Ivory Coast Ivory Coast SLEV/JEVWNV/TBEV DENV1-4 neg neg TARGET Score* Classification
16a TaqMan RT-PCRa + + + + + + + + + (−) − − − − YFV 26 OPTIMAL
8 TaqMan RT-PCRb + + + + (−) + + + + (−) − − − − PANFLAV 24 OPTIMAL
17b RT nested PCRc + + + + + + + + + + − (+) − − PANFLAV 24 NON OPTIMAL
1 TaqMan RT-PCRa + + + + + + + + + + − (+) − − YFV 24 NON OPTIMAL
27 TaqMan RT-PCRd + + + + + + + + + + − (+) − − YFV 24 NON OPTIMAL
28 TaqMan RT-PCRe + + + + + + + + + + − (+) − − YFV 24 NON OPTIMAL
15 TaqMan RT-PCRa + + + + + + + + + (−) − (+) − − YFV 22 NON OPTIMAL
17a TaqMan RT-PCRf + + + + + + + + + (−) − (+) − − YFV 22 NON OPTIMAL
6 RT nested PCRx + + (−) (−) (−) + + + + (−) − − − − PANFLAV 20 OPTIMAL
22a RT nested PCRg + + ND + (−) + + (−) (−) + − − − − PANFLAV 20 OPTIMAL
16b Heminest Rt-PCRc + + + + + + + + + (−) − (+) − − PANFLAV 20 NON OPTIMAL
2 TaqMan RT-PCRa + + + + (−) + + + + (−) − (+) − − YFV 20 NON OPTIMAL
9 TaqMan RT-PCRa + + + + (−) + + + + (−) − (+) − − YFV 20 NON OPTIMAL
14 RT nested PCRg + + + (−) (−) + (−) + (−) (−) − − − − PANFLAV 18 OPTIMAL
4 TaqMan RT-PCRa + + + + (−) + + + (−) (−) − (+) − − YFV 18 NON OPTIMAL
10 TaqMan RT-PCRe + + + + + (−) (−) (−) (−) (−) − (+) − − YFV 14 NON OPTIMAL
11 RT-PCRh + + + + + (−) (−) (−) (−) (−) − (+) − − YFV 14 NON OPTIMAL
20 Heminest RT-PCRc + + + + + + + + + + (+) (+) − (+) PANFLAV 16 NON OPTIMAL
3b TaqMan RT-PCRe + + + + + (−) (−) (−) (−) (−) − (+) − − YFV 14 NON OPTIMAL
5 TaqMan RT-PCRx + + + + + (−) (−) (−) (−) (−) − (+) − − YFV 14 NON OPTIMAL
13 TaqMan RT-PCRa + + ND + + (−) (−) + (−) (−) − (+) − − YFV 14 NON OPTIMAL
18 TaqMan RT-PCRe + + + + + (−) (−) (−) (−) (−) (+) − − − YFV 14 NON OPTIMAL
3a RT nested PCRf + + + (−) (−) + (−) (−) (−) (−) − (+) − − YFV 12 NON OPTIMAL
19 TaqMan RT-PCRa + + + (−) (−) + (−) (−) (−) (−) − (+) − − YFV 12 NON OPTIMAL
22b TaqMan RT-PCRe + + ND + + (−) (−) (−) (−) (−) − (+) − − YFV 12 NON OPTIMAL
7 TaqMan RT-PCRi + + + + + + (−) (−) (−) + (+) (+) − (+) YFV 10 NON OPTIMAL
21 TaqMan RT-PCRe + + + (−) (−) (−) (−) (−) (−) (−) − (+) − − YFV 10 NON OPTIMAL
25 RT-nested PCRx + (−) (−) (−) (−) ND (−) (−) ND (−) − − − − YFV 8 NON OPTIMAL
12 SYBR-RT-PCRb + + + (−) (−) + (−) (−) + (−) (+) (+) (+) − PANFLAV 6 NON OPTIMAL
23 RT-PCRj + + + (−) (−) + (−) (−) (−) (−) − (+) − − PANFLAV 0 NON OPTIMAL
24 RT-nested PCRk (−) (−) (−) (−) (−) (−) (−) (−) (−) (−) − − − − YFV NS NON OPTIMAL
26 RT-nested PCRf + (−) (−) + (−) (−) + (−) + + (+) (+) (+) (+) YFV NS NON OPTIMAL
CORRECT RESULTS (%) 96.8 90.6 86.2 71.8 53.1 67.7 50 50 48.3 25 84.3 25 93.7 93.7
*

The score reflects the quality of the diagnostic results: +, positive result (score 2 points); (+), false positive (score -2 points); −, correct negative result (score 2 points); (−), false negative (score 0 points).

a

[20];

b

adapted from [23];

c

[19];

d

[24];

e

[21];

f

in-house;

g

[18];

h

[22];

i

[16];

j

[15];

k

[17];

x

not provided.

Sequencing performed; NS: Negative Score; ND: not done; PANFLAV: panflavivirus; YFV: yellow fever virus.