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BrdU uptake assay of granulosa cells by immune-fluorescence analysis. Granulosa cells synchronized at G1/S interphase were treated with 50 μmol/L SP600125 and AS601245 and BrdU uptake was compared between control and JNK inhibitor groups; 17% and 21 % of cells treated with 50 μmol/L SP600125 and AS601245, respectively, were BrdU positive compared to 55% of control cells (P < .001). JNK indicates c-Jun N-terminal kinase.
