Figure 6. The E3 ligases Hel1, Hel2, Pep5, Snt2 and Tom1 do not regulate the protein levels of Ubc4, the major E2 enyme involved in excess histone degradation.

Whole cell lysates were prepared as described previously [69] from wild type and the indicated E3 ligase deletion strains carrying MYC-tagged Ubc4. The lysates were resolved on a 12% polyacrylamide gel and processed for Western blotting using MYC antibodies. No significant differences are observed in the levels of full-length Ubc4-MYC, or in its slower migrating modified forms and faster migrating degradation products.