Skip to main content
. 2012 Apr 26;8(4):e1002665. doi: 10.1371/journal.pgen.1002665

Figure 3. The TIAM-1 DHPH domain is a Rac1-specific GTP exchange factor.

Figure 3

Graphs plotting arbitrary fluorescence units (A.U.) over time in a mant-GTP exchange assay (Cytoskeleton, Inc.) (see Methods). Dark, cross-hatched points represent exchange activity of the GTPase after the addition of vehicle without the DHPH domain of TIAM-1. Light, diamond-shaped points represent exchange after the addition of the DHPH domain of TIAM-1 in vehicle. The dashed vertical line represents the time of addition of vehicle or vehicle plus TIAM-1 DHPH. A) Exchange activity on human Rac1. B) Exchange activity on human RhoA. C) Exchange activity of human Cdc-42. D) Exchange activity on human Rac1 of a TIAM-1 DHPH domain with the T516F point mutation predicted to eliminate Rac GEF activity (TIAM-1(DEAD)). Error bars represent standard deviations of three independent experiments which each had consistent results.