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. 2012 Apr 26;8(4):e1002670. doi: 10.1371/journal.pgen.1002670

Figure 1. Dma proteins are required together with Cla4 for septin ring deposition.

Figure 1

A–B: Logarithmically growing cultures of wild type (ySP3070), dma1Δ dma2Δ (ySP4319), cla4-75 (ySP4320) and dma1Δ dma2Δ cla4-75 (ySP4321) cells, all expressing a fusion of the Cdc3 septin with GFP, were arrested in G1 by alpha factor at 25°C and released into the cell cycle at 37°C. At the indicated times, cell samples were taken for FACS analysis of DNA contents (A) and for scoring budding, nuclear division and septin ring deposition (B). C: Septin rings labelled by GFP-Cdc12 were subjected to FRAP using the following strains: ySP8176 (wt), ySP8173 (dma1Δ dma2Δ), ySP8296 (cla4-75) and ySP8193 (dma1Δ dma2Δ cla4-75). Half of the ring was bleached at time 0. Recovery of fluorescence was measured over time upon shift to 37°C and plotted as fraction of fluorescence intensity relative to time 0 (unbleached). Bars indicate standard errors.