Figure 2.
Effects of ovarian steroid hormone treatment on histone deacetylase (HDAC) 1/2 gene expression in human endometrial and endometriotic cell lines. Cells were treated for 24 hours with 10−8 mol/L estradiol (E2), 10−7 mol/L progesterone (P4), or E2/P4 for 24 hours. Total RNA was isolated from treated and untreated (medium with vehicle, CV) cells. Quantitative polymerase chain reaction (qPCR) of HDAC1/2 was conducted using TaqMan gene expression assays. Results are expressed as relative expression (relative units, RU) after normalization with the reference gene. The HDAC1 gene expression was significantly downregulated by E2/P4 in endometrial epithelial cells (B) but not in endometrial stromal nor in the endometriotic cells (C). The HDAC2 gene expression levels were significantly increased by E2 and dowregulated by E2 + P4 in endometrial stromal cells (A). **P < .01.