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. 2012 Apr 26;8(4):e1002686. doi: 10.1371/journal.pgen.1002686

Figure 6. LIC and BZR1 expression patterns and their responses to BR.

Figure 6

(A) RNA in situ expression of LIC and BZR1 on the abaxial and adaxial sides of leaves (the bottom panel represents the negative control with sense probes). Bar = 10 µm. (B) LIC and BZR1 transcriptional expression response to various concentrations of BR. Data are mean ± SD (n = 5). *P<0.05 and **P<0.01 compared with no BR treatment as determined by Student's t test. (C) Immunoblotting to show the response of LIC protein expression to BR. LIC was repressed by low concentrations of BR (<100 nM) and induced by high concentrations of BR (>200 nM). Coomassie Blue staining served as the loading control. The levels of LIC were calculated after normalization against the intensity of Coomassie Blue staining in 3 replicated experiments, and the quantified values are shown beneath the gel images. Data are mean ± SE. (D) LIC transcriptional expression with BR treatment in wild-type (WT) and BR-deficient mutant d2 and BZR1 RNAi transgenic lines (BZR1R). LIC antisense line 2 (AS2) was a control. Data are mean ± SD (n = 3). (E) Time course response of transcription expression of LIC to BR (1 µM). LIC was rapidly induced by BR. Data are mean ± SD (n = 3). (F) BZR1 and CPD transcriptional response to BR treatment in the wild type and LIC antisense lines. For BZR1, data are mean ± SD (n = 5). *P<0.05, compared with no BR treatment. For CPD, Data are mean ± SD (n = 3).