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. 2012 Apr 26;8(4):e1002683. doi: 10.1371/journal.pgen.1002683

Figure 5. dZip1 knockdown significantly lengthens the lifespan of Aβ42 flies.

Figure 5

The percentage of survivorship was plotted against the age (dae). dZip1 OE significantly shortened the life span of Aβ42 (elav-Gal4>UAS-Aβ42) flies with a 12.5% and a 22.2% reduction in the median lifespan reared at 25°C (p<0.001, A and D) and 29°C (p<0.001, B and D), respectively. dZip1 RNAi significantly prolonged the life span of elav-Gal4>UAS-Aβ42 flies, in which dZip1-RNAi 1# (elav-Gal4>UAS-Aβ42/UAS-dZip1-RNAi 1#) flies had a 11.4% and 27.7% increase in the median lifespan over that of the Aβ42 flies at 25°C (p<0.001, A and D) and 29°C (p<0.001, B and D), respectively, and dZip1-RNAi 2# (elav-Gal4>UAS-Aβ42/UAS-dZip1-RNAi 2#) flies had a 33.3% and a 88.8% increase in the median lifespan over that of the Aβ42 flies at 25°C (p<0.001, A and D) and 29°C (p<0.001, B and D), respectively. Without Aβ42 expression, the median lifespans of dZip1-RNAi 1# and 2# flies were the same as that of the control elav-Gal4 flies (C and D), but that of the dZip1 OE flies showed a 7.2% reduction (p<0.001, C and D) when raised at 25°C. Differences shown are all statistically significant (p<0.001). Reported P values are from Mantel-Cox log-rank statistical analysis.