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. 2012 Apr 26;8(4):e1002486. doi: 10.1371/journal.pcbi.1002486

Figure 5. Hdac1 regulates expression of LTR retrotransposons in mouse ES cells.

Figure 5

(A) qRT-PCR verification of LINE-1, RLTR45 and IAP expression in Hdac1−/− ES cells. Expression levels (mean ± standard error for three biological replicates) were normalized to β-Actin and expressed relative to control ES cells. IAP and LINE1 5′UTR primer sets (Figure S2) were used to assess IAP and LINE-1 expression. Asterisks indicate a statistically significant difference (p<0.05) for RLTR45 and IAP elements. RLTR45 expression is upregulated in Hdac1−/− ES cells, but IAP expression is downregulated. (B) Enrichment of LTR retrotransposon sequences in Hdac1 ChIP-seq data from mouse ES cells. The maximum likelihood of enrichment (±95% confidence intervals) for RLTR45 LTR and IAP LTR sequences Hdac1 ChIP-seq relative to whole cell extract is shown. RLTR45 LTR sequences are enriched in the Hdac1 ChIP-seq indicating a physical association between Hdac1 and RLTR45 retrotransposon chromatin, in contrast IAP LTR sequences are depleted.