Figure 9.

5-Hydroxytryptamine (5-HT)-dependent Ca²⁺ signals recorded in situ are reduced by antenatal maternal LTH in pulmonary arterial myocytes of fetal sheep. A, Bars indicate the average number of myocytes with Ca²⁺ responses each minute in the absence (open) and presence (solid) of 10 μmol/L 5-HT. B, Bars indicate the average time period in between Ca²⁺ events in the absence (open) and presence (solid) of 10 μmol/L 5-HT. Error bars represent ± SEM. ####P < .0001 denotes significant difference from normoxic counterparts, while **P < .01 or ****P < .0001 denotes significant difference from control based on a 2-way ANOVA and a Bonferroni post hoc analysis. LTH indicates long-term hypoxia; SEM, standard error of the mean; ANOVA, analysis of variance.