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. 2012 May 3;7(5):e36233. doi: 10.1371/journal.pone.0036233

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Juri Ayub et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Crude extracts from T. cruzi (lane 1), T. brucei (lane 2), C. fasciculata (lane 3) and R. norvegicus (lane 4) were analyzed by SDS-PAGE and Western blot with scFv C5. The position of previously characterized P proteins from T. cruzi (Tc) is shown on the left. The image is representative of three independent assays. B. Dose-response effect of scFv C5 on protein synthesis by ribosome extracts from R. norvegicus and T. cruzi. C. Effect of scFv C5 160 nM on in vitro protein synthesis in ribosome extracts from R. norvegicus, C. fasciculata and T. cruzi compared with the translation inhibitor emetine at 0.1 mg/mg. Average values for control assays were 80,000 cpm, 48,000 cpm and 43,000 cpm for R. norvegicus, C. fasciculata and T. cruzi, respectively. The scFv C5 significantly inhibited protein synthesis by C. fasciculata and T. cruzi ribosomes (*, p<0.05; ***, p<0.001). D. Effect of the preincubation with R13 or H13 peptide on the translation inhibition by scFv C5 (**; p<0.01).