Figure 3.

Interleukin 1 (IL-1) signaling did not decrease lipopolysaccharide (LPS)-induced chorioamnion pro-inflammatory messenger RNA (mRNA) expression in preterm fetal sheep. Assessments were made 2 days after exposures. Quantification of (a) IL-1β, (b) IL-6, (c) IL-8, or (d) MCP-1 mRNAs in the chorioamnion. The mRNA quantitations were performed using real-time PCR with Taqman probe assays and values were normalized to 18S ribosomal protein mRNA (internal control), and levels for each animal group were expressed relative to controls. Inhibition of IL-1 signaling did not change LPS-induced increases in the inflammatory genes (n = 4-5 animals/group; * P < .05 compared to controls).