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. Author manuscript; available in PMC: 2012 Nov 3.
Published in final edited form as: Nature. 2012 Mar 29;485(7396):62–68. doi: 10.1038/nature11030

Figure 2. Synthetic REV-ERB ligands alter circadian behavior and the pattern of expression of core clock genes.

Figure 2

a, Actograms illustrating the effect of single injections of vehicle, SR9011 (100 mg/kg, i.p.) or SR9011 (100 mg/kg, i.p.) on circadian behavior. C57Bl6 mice were initially maintained on a 12h:12h L:D cycle and altered to constant darkness (D:D) after 7-days. After 12 days on D:D the animals were injected with vehicle or compound at CT6. b, Analysis of wheel running activity during the subject dark period following injection of SR9011 i.p. at CT6 in mice kept under constant darkness. c, Normalized expression levels of several core clock genes following administration of SR9011 or vehicle under constant dark conditions. C57Bl6 mice were administered SR9011 (100 mg/kg, i.p.) at CT0 on a day of constant darkness. Gene expression was determined and normalized to cyclophilin. Data were double plotted. d, Actograms illustrating the effect of single injections of vehicle, SR9011, or SR9009 in mice maintained under 12:12 L:D conditions. e, Normalized expression levels of several core clock genes following administration of SR9011 or vehicle under L:D (12:12) conditions. Methods for e and e were otherwise identical to a and c. * indicates p<0.05. Error bars indicate mean ± s.e.m. and n=6–10 mice.