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Detection of glomerular (a) IgG2b, 163p.77 but not (b) IgG2a, 452s.160 in kidney serial cryosections 24 hours after co-injecting 1 mg of each purified mAb into a BALB/c mouse. Serial cryosections had granular IgG2b but no IgG2a within MM. Mice injected with 163p.64, IgG2a and 452s.46, IgG2b (see Fig. 3a and c) and 163p.124, IgG2a and 163p.132, IgG2b had IgG2a but no IgG2b staining. Results were similar in replicate mice.
