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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Kidney Int. 2012 Feb 1;82(2):184–192. doi: 10.1038/ki.2011.484

Table 3.

Hybridomas producing BM-binding mAb induce proteinuria.

Hybridoma(s) Injected mAb Isotype Glomerular Isotypeb Daysc Anti-DNA Serum Titer Proteinuria
(2a/2b)d (mg/dl)e
163p.64 2a 2a 4 1 601/<90 100
2a 5 11 842/<90 300
163p.77 2b 2b 5 <90/36 000 100
163p.124 2a 2a 5 24 000/<90 100
DNA5 2a 2a 5 36 000/<90 100
DNA6 2a 2a 5 36 000/<90 100
452s.46 2b None 5 <90/28 024 <30
DNA3 2a None 5 36 000/<90 <30
163p.132 2b ~2bf 5 <90/32 938 <30
2b 8 <90/>200 000 100
163p.64 2a 2a 4 2 578/4 546 100
163p.132 2b 2a 5 24 704/25 202 300
163p.77 2b 2b 5 36 000/36 000 30
DNA3 2a
163p.124 2a 2a 5 24 000/24 000 100
163p.132 2b
163p.64 2a 2a 5 12 001/41 470 100
452s.46 2b
a

Ten mice per group were injected with the indicated hybridomas on day 0 and monitored daily for proteinuria. Two mice per group were terminated daily. Results are presented from one mouse in each group. Similar results were obtained with the other mouse in each group on the respective day.

b

The subclass of IgG detected within kidney serial cryosections was determined by immunofluorescence from kidneys excised on the indicated days after hybridoma injection.

c

The number of days after injection of hybridoma cells.

d

Serum IgG2a and IgG2b anti-DNA titers were determined on the indicated days after hybridoma injection.

e

Proteinuria measured on the indicated days after hybridoma injection.

f

Weak immunofluorescence only slightly above background. Two mice were separately injected with 163p.132 in a later experiment. Sera and kidneys were collected and proteinuria was measured 8 days after hybridoma injection. See Fig. 3f.