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Primer sequences used for qRT-PCR
| Gene | Primer Sequence (5′ to 3′) | NT |
|---|---|---|
| 18S | GCATGGCCGTTCTTAGTTGGTGGA | 24 |
| TCTCGGGTGGCTGAACGCCA | 20 | |
| Snai1 | CACCTCCAGACCCACTCAGAT | 21 |
| CCTGAGTGGGGTGGGAGCTTCC | 22 | |
| MMP9 | GCCCACCGTCCTTTCTTGTTGGA | 23 |
| GGGAGAGGTGGTTTAGCCGGTG | 22 | |
| Twist | CCACGCTGCCCTCGGACAAG | 20 |
| CCAGGCCCCCTCCATCCTCC | 20 | |
| Vimentin | CCAGAGACCCCAGCGCTCCT | 20 |
| GCCGGAGCCACCGAACATCC | 20 | |
| E-cadherin | TTGAGGAGTTGAATGCTGAC | 20 |
| AGCTCGAACTTTCCAAGCAG | 20 | |
| Kiss1 | GCAAGCCTGGGTCTGCAGGG | 20 |
| CGACTGCGGGAGGCACACAG | 20 |
