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. Author manuscript; available in PMC: 2012 Nov 3.
Published in final edited form as: Nature. 2012 Apr 8;485(7396):128–132. doi: 10.1038/nature10988

Figure 2.

Figure 2

Glucagon stimulates CRTC2 dephosphorylation via activation of InsP3 receptors. A. Effect of glucagon (Gcg) on calcium mobilization in hepatocytes by fluorescence imaging. Calcium mobilization and calcineurin activation following knockdown of all three InsP3R family members shown (*P < 0.001; n=3). B. Effect of calcium chelator (BAPTA) on CRTC2 dephosphorylation and CRE-luc activation (*P < 0.001; n=3). C. Effect of InsP3R depletion on CRTC2 dephosphorylation, CRE-luc activity, and glucose output from hepatocytes (*P < 0.001; n=3). D. Effect of hepatic InsP3R knockdown on CRE-luc activity, blood glucose, and gluconeogenic gene expression (*P < 0.01; n=5).