Figure 4.
InsP3R activity is upregulated in diabetes. A. Hepatic CRE-luc and calcineurin activity in lean and db/db mice (*P < 0.001; n=5). B. Immunoblots showing relative amounts and phosphorylation of InsP3R family members in livers of ad libitum fed lean, db/db, or ob/ob mice. InsP3R phosphorylation at PKA or AKT sites indicated. C. Effect of RNAi-mediated depletion of InsP3Rs or calcineurin A on CRE-luc activity, gluconeogenic gene expression, and hepatic glucose production in db/db mice, determined by pyruvate tolerance testing (*P < 0.01; n=5).