Fig. 2.

Increased density of gelatin promotes trophoblast matrix degradation. (A) Typical single point degradation levels by trophoblasts on a 0.2% fluorescent gelatin matrix. (B) Degradation of gelatin by trophoblast cells on 1% (w/v) gelatin is seen to be greater than in (A) (0.2%, w/v gelatin) over the same incubation period. (C) Quantification of the matrix degraded shows a significant increasing trend for the degraded area and degrading intensity for trophoblast cells on increasing concentrations of gelatin. Percentage of degrading cells does not differ with trophoblast cells on 0.2% gelatin close to 100%. * P < 0.05, **P < 0.01 and ***P < 0.001. Arrow heads indicate a single actin rich protrusion. All scale bars equal to 10 μm.