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. 2012 Jan;2(1):53–67. doi: 10.1002/brb3.33

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© 2012 The Authors. Published by Wiley Periodicals, Inc.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Defining an optimal condition for myelination cultures from E16 rat spinal cord. Cultures maintained in N2 showed poor density of neurite (A), high density of mature OLs (B), but very few myelin segments (arrow heads in C). In contrast, NBM showed a markedly increased neurite density (D), but slight decrease of mature OLs (E), and a moderate increase of myelin segments (F), when compared to N2. A defined medium by mixture of N2 and NBM (1:1) resulted in optimized neurite growth (G), better OL differentiation as shown by highly branched premyelinating OLs (H), and significantly increased number of myelin segments (I). Myelin basic protein (MBP) was downregulated in OL cell bodies at this stage (arrows in F and I). Scale bar: 100 μm (A, D, and G); 25 μm (B, C, E, F, H, and I).