Figure 6.

Myelination was significantly increased in the cortex-derived culture by accelerating OL maturation. Three days after T3 was introduced into the medium, the number of NG2+ (A) was reduced while O4+ (B) and myelin basic protein (MBP)+ OLs (C) increased significantly compared to cultures without T3 (see Fig. 5). At DIV 26, active myelination was noted (D), and by DIV40, abundant myelin was formed in the culture (E). The nodes of Ranvier were also evidenced by the clustering of Caspr immunoreactivity (F). Scale bar: 25 μm (A–E); 10 μm (F).