Table 1.
Antibodies used in immunocytochemistry in this study
| Antibody name | Source | Dilution | Host | Target(s) of labeling |
|---|---|---|---|---|
| Myelin basic protein (MBP) | Chemicon | 250 | Ms | Myelin; mature OL |
| Neural/Glial antigen 2 (NG2) | Chemicon | 400 | Rb | Early OL progenitor |
| OL marker O4 | Chemicon | 800 | Ms | Late OL progenitor |
| OL transcription factor 2 (Olig2) | Chemicon | 500 | Rb | OL lineage (progenitor, immature, and mature OL) |
| Neuronal nuclei (NeuN) | Chemicon | 400 | Ms | Mature neuron |
| CD11b | Chemicon | 400 | Ms | Microglia/macrophage |
| Glial fibrillary acidic protein (GFAP) | Chemicon | 800 | Rb | Astrocyte |
| Contactin-associated protein (Caspr) | Chemicon | 200 | Rb | Paranodal domain |
| Potassium channel Kv1.2 | Abcam | 200 | Rb | Juxtaparanodal domain |
| Phosphorylated neurofilament H (pNF) | Convance | 1000 | Rb | Mature axon |
| β-III tubulin (Tuj1) | Sigma | 1200 | Ms | Neurite (axon and dendrite) |