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. Author manuscript; available in PMC: 2012 May 4.
Published in final edited form as: Placenta. 2008 Nov 25;30(1):66–73. doi: 10.1016/j.placenta.2008.10.009

Figure 3.

Figure 3

Two proteins, CTGF/CCN2 and JunB, increase similarly to the increase in their mRNA transcripts 24hr. TSC were cultured without stress (A, B; A’, B’), for 24hr with stress (C, D; C’, D’) and probed with antibodies to CTGF/CCN2 or JunB as indicated. TSC were cultured for 24hr of 400mM sorbitol and then probed with no first antibody (E, F; E’, F’). Paired micrographs in the first and second and in the third and fourth columns show protein immunofluorescence and Hoechst-stained nuclei, respectively. At the bottom of each micrograph series is an immunoblot derived from TSC cultured without stress or after 24hr of 400mM sorbitol. Immunoblots were probed with antibodies to CTGF/CCN2 or JunB and then reprobed with antibodies to Actin (ACTB) as a loading control. Bands for CTGF/CCN2 and JunB are both detected at the correct size of 38kDa.