Figure 5.

Brf is required for TOR-induced cell growth in both mitotically dividing and endoreplicating tissue in Drosophila larvae. (A–F) brf, tsc1 or tsc1,brf double mutant clones were induced in both wing discs (A–C) and fat body (D–G). Mutant clones, arrowheads; wild-type sister clones, arrows. Blue, DAPI staining; red, actin; green, GFP. (G) The areas of both mutant and wild-type cells in the fat body were measured and presented here as mean cell area compared with control. Genotypes: (A, D) hsflp¹²²; +; FRT82B, brf^EY02964/FRT82B, ubi-GFP; (B, E) hsflp¹²²; +; FRT82B, tsc1^Q87X/FRT82B, ubi-GFP; (C, F) hsflp¹²²; +; FRT82B, brf^EY02964, tsc1^Q87X/FRT82B, ubi-GFP. (A–C) Scale bar, 50 μm. (D–F) Scale bar, 100 μm.