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. 2012 Mar 6;31(8):1879–1892. doi: 10.1038/emboj.2012.43

Figure 2.

Figure 2

Cyclin D2 mRNA contains a cis-acting transport element in the 3′ untranslated region. In-situ hybridization for EGFP (A) and RFP mRNA (B), coded by pCE/CD2/3′ and pCAGGS-mRFP, respectively, following electroporation at E12.5. EGFP mRNA is found in the VZ as well as in the basal process and the basal endfoot of the radial glia (arrowheads in A). In contrast, RFP mRNA without Cyclin D2 3′UTR is only found in the VZ without expression in the basal process (B). (C) Identification of a 50 nucleotide cis-acting mRNA transport element in the Cyclin D2 3′ UTR. Left panel, regions of the Cyclin D2 3′ UTR fused with EGFP ORF, right panel shows localization of EGFP-CyclinD2 3′UTR chimeric mRNA in the mouse diencephalon. (D) Diagrammatic representation of Cyclin D2 mRNA and cis-acting transport element (3965–4015). VZ, ventricular zone. Scale bar: 100 μm.