Figure 9.

Relative timescales of IAPP assembly kinetics under different solution conditions. IAPP kinetics were monitored over time with the change in fluorescence of the exogenous dye ThT. Kinetic profiles were fit to a sigmoid to extract the time at half-maximum intensity (t₅₀). All reactions were measured on 20 μM hIAPP in standard conditions with 50 μM ThT and at 30°C. t₅₀ values were measured with 1 M urea, 20 mM bicelles, 5 mM liposomes, or 2% TFE and reported relative to the t₅₀ in standard buffer conditions. All values reported are the mean for triplicate reactions ± one stdev. (Inset) Representative IAPP kinetic data (squares) showing the fit (line) and t₅₀ value (dashed line).