Skip to main content
. 2012 May;139(5):371–388. doi: 10.1085/jgp.201210770

Figure 4.

Figure 4.

Representative raw SV data at different GluA2 concentrations measured using absorbance and fluorescence detection. (A) Data for GluA2S measured by absorbance at 230 nm. (B) Data for EndoH-digested FAM-labeled GluA2S measured using fluorescence detection; the data shown form part of the sw isotherm shown in Fig. 3 D. In both panels, the radial signal distributions are shown at equidistant time points after start of the centrifugation, with later times indicated by higher color temperatures on a blue < green < red scale. (C) c(s) distribution obtained from the analysis of the 11-nM data of GluA2S shown in A. Integration and Monte-Carlo analysis leads to an sw value of 4.46 S (4.16–4.56 S). (D) For comparison, analogous c(s) distribution of FAM-labeled GluA2S at 7.4 nM derived from FDS data, leading to an sw value of 4.14 S (4.06–4.26 S).