Fig. (6).

Network of thin nanotubular connections (indicated by black arrows) between negatively charged POPC-cholesterolcardiolipin giant unilamellar vesicles (GUVs) in the presence of β2- GPI (100 mg/L) and serum IgG antibodies (75 mg/mL) from an antiphospholipid syndrome patient, containing antibodies against β2-GPI. GUVs were observed under Zeiss Axiovert 200 Phase Contrast Microscope (Zeiss, Germany), magnification 1000 x in 0.2 mol/l sucrose/glucose/ PBS solution; pH 7.4; T_v =37°C; ionic strength 10 mmol/l. POPC:cholesterol: cardiolipin mass proportionin GUVs = 7:2:1. (adapted from [38]).