FIGURE 2.
Cytokine detection was assayed in the lung tissue from BALB/c mice 52 days after infection. Analyzed cytokines were: (A) IFN-γ, (B) TNF-α, (C) IL-4, and (D) IL-10. Each group was infected i.t. with 3 × 105 yeast cells and immunized at 30, 37, and 44 days after infection with different adjutants with or without P10.The groups of mice included unimmunized, infected control mice (INF); animals infected and immunized with aluminum hydroxide alone (ALU) or with P10 (AP10), FliC flagellin alone (FLA) or with P10 (FP10), and cationic lipid alone (CLI) or with P10 (CP10). *p < 0.05: significance p < 0.05 compared to control mice (only infected). **p < 0.01: compared to control mice (only infected).