Figure 4.

ATP binding favours jaw tightening. (A) Schematic representation of the heteromeric expression of K69A/T339S and H213A/T339S mutants (see text for details). (B) Representative traces of Zn²⁺ (200 μM)-evoked currents potentiated by 0.1 μM ATP recorded from cells expressing the heteromer (black), the K69A/T339S homomer (blue) or the H213A/T339S homomer (orange). (C) Pooled data for each of the three constructions shown in B (n=4–7). Potentiation was defined as the ratio of currents recorded in the presence of both ATP and zinc to those recorded in the presence of zinc alone. ND, not determined, because no Zn²⁺ inward current was detected in cells expressing the mutant H213A/T339S.