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. 2001 May 8;98(11):6138–6143. doi: 10.1073/pnas.111139598

Table 1.

Oligonucleotides used for RT-PCR, preparation of recombinant proteins, and EMSA

Names Sequences
Group 1
 606 5′-AGTTAAAGCAGAACTGCGT-3′
 741 5′-CAGAGGGTATCTTCTGGAC-3′
 swk2s 5′-GTAGAGTTGGTTGCTATAGT-3′
 mo4a 5′-TTAGGATC CTCTTGTAAGACTCCCTAAT-3′
Group 2
 ror2s 5′-AGACGCGGATCC TCGTATGTTGTGCCTGATCT-3′
 ror2a 5′-CCGCTAGAATTCTTA TGTAGCAGGTTCTGA TGACT-3′
 ro2is 5′-ATTCGAGGATCC AAACGGATTGGTTATATATG-3′
 ro2ia 5′-GTCTCAGAATTC TTAATTAAAATTTTTCAGAT-3′
 ror1s 5′-TAACGCGGATCC GCAAATCTGAAGTCTGAAAA-3′
 ror1a 5′-GTTCTAGAATTCTCA CCAGGTTTTGGAAGTGTT-3′
 ror1a2 5′-GTTCTAGAATTCTCA TGGGGG ACCAATCTG-3′
 ror1va 5′-GTTCTAGAATTCTCA GAGAAATAGTTTCACTT-3′
Group 3
 GASs 5′-GATTTTCCCAGAAAAGGAAC-3′
 ISREs 5′-TTTACAAACAGCAGGAAATAGAAACTTAAGAGAAATACA-3′

Group 1 was used for RT-PCR detection of ovIFNAR1f and ovIFNAR1e; group 2 was used for preparing recombinant proteins; and group 3 (sense only shown) for EMSA.