Table 2.

The AV activities of soluble IFNAR proteins

Proteins	AV activities, nM
GST-R2E	126
R2E*	14
R2E†	12
GST-R1e	3,300
R1e*	1,100
GST-R1E	1,000
GST-R1SD1	3,000

Insoluble proteins from inclusion bodies were solubilized with 8 M urea and refolded in 50 mM Tris⋅HCl, pH 8.0 and 100 mM NaCl. Data are averages from three experiments, each carried out in duplicate and performed within 2 weeks of refolding the proteins (which were stored at 4°C). The GST-R2E corresponds to preparation 1 in Table 3. All assays (based on a series of 1∶3 dilutions) gave identical results. Hence, there are no error terms. 

^*Produced from GST-fusion protein. 

^† Produced with pET15b vector without GST.